Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered Lacticaseibacillus rhamnosus CRL1505.
Identifieur interne : 000217 ( Main/Exploration ); précédent : 000216; suivant : 000218Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered Lacticaseibacillus rhamnosus CRL1505.
Auteurs : Valeria Garcia-Castillo [Argentine, Japon] ; Mikado Tomokiyo [Japon] ; Fernanda Raya Tonetti [Argentine] ; Md Aminul Islam [Japon] ; Hideki Takahashi [Japon] ; Haruki Kitazawa [Japon] ; Julio Villena [Argentine, Japon]Source :
- Frontiers in immunology [ 1664-3224 ] ; 2020.
Abstract
The oral administration of Lacticaseibacillus rhamnosus CRL1505 differentially modulates the respiratory innate antiviral immune response triggered by Toll-like receptor 3 (TLR3) activation in infant mice, improving the resistance to Respiratory Syncytial Virus (RSV) infection. In this work, by using macrophages depletion experiments and a detailed study of their production of cytokines and antiviral factors we clearly demonstrated the key role of this immune cell population in the improvement of both viral elimination and the protection against lung tissue damage induced by the CRL1505 strain. Orally administered L. rhamnosus CRL1505 activated alveolar macrophages and enhanced their ability to produce type I interferons (IFNs) and IFN-γ in response to RSV infection. Moreover, an increased expression of IFNAR1, Mx2, OAS1, OAS2, RNAseL, and IFITM3 was observed in alveolar macrophages after the oral treatment with L. rhamnosus CRL1505, which was consistent with the enhanced RSV clearance. The depletion of alveolar macrophages by the time of L. rhamnosus CRL1505 administration abolished the ability of infant mice to produce increased levels of IL-10 in response to RSV infection. However, no improvement in IL-10 production was observed when primary cultures of alveolar macrophages obtained from CRL1505-treated mice were analyzed. Of note, alveolar macrophages from the CRL1505 group had an increased production of IL-6 and IL-27 suggesting that these cells may play an important role in limiting inflammation and protecting lung function during RSV infection, by increasing the maturation and activation of Treg cells and their subsequent production of IL-10. In addition, we provided evidence of the important role of CD4+ cells and IFN-γ in the activation of alveolar macrophages highlighting a putative pathway through which the intestinal and respiratory mucosa are communicated under the influence of L. rhamnosus CRL1505.
DOI: 10.3389/fimmu.2020.568636
PubMed: 33133080
PubMed Central: PMC7550464
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered <i>Lacticaseibacillus rhamnosus</i> CRL1505.</title><author><name sortKey="Garcia Castillo, Valeria" sort="Garcia Castillo, Valeria" uniqKey="Garcia Castillo V" first="Valeria" last="Garcia-Castillo">Valeria Garcia-Castillo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 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uniqKey="Raya Tonetti F" first="Fernanda" last="Raya Tonetti">Fernanda Raya Tonetti</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation></author><author><name sortKey="Islam, Md Aminul" sort="Islam, Md Aminul" uniqKey="Islam M" first="Md Aminul" last="Islam">Md Aminul Islam</name><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Takahashi, Hideki" sort="Takahashi, Hideki" uniqKey="Takahashi H" first="Hideki" last="Takahashi">Hideki Takahashi</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Plant Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Plant Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Kitazawa, Haruki" sort="Kitazawa, Haruki" uniqKey="Kitazawa H" first="Haruki" last="Kitazawa">Haruki Kitazawa</name><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Villena, Julio" sort="Villena, Julio" uniqKey="Villena J" first="Julio" last="Villena">Julio Villena</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33133080</idno><idno type="pmid">33133080</idno><idno type="doi">10.3389/fimmu.2020.568636</idno><idno type="pmc">PMC7550464</idno><idno type="wicri:Area/Main/Corpus">000019</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000019</idno><idno type="wicri:Area/Main/Curation">000019</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000019</idno><idno type="wicri:Area/Main/Exploration">000019</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered <i>Lacticaseibacillus rhamnosus</i> CRL1505.</title><author><name sortKey="Garcia Castillo, Valeria" sort="Garcia Castillo, Valeria" uniqKey="Garcia Castillo V" first="Valeria" last="Garcia-Castillo">Valeria Garcia-Castillo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Tomokiyo, Mikado" sort="Tomokiyo, Mikado" uniqKey="Tomokiyo M" first="Mikado" last="Tomokiyo">Mikado Tomokiyo</name><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Raya Tonetti, Fernanda" sort="Raya Tonetti, Fernanda" uniqKey="Raya Tonetti F" first="Fernanda" last="Raya Tonetti">Fernanda Raya Tonetti</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation></author><author><name sortKey="Islam, Md Aminul" sort="Islam, Md Aminul" uniqKey="Islam M" first="Md Aminul" last="Islam">Md Aminul Islam</name><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Takahashi, Hideki" sort="Takahashi, Hideki" uniqKey="Takahashi H" first="Hideki" last="Takahashi">Hideki Takahashi</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Plant Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Plant Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Kitazawa, Haruki" sort="Kitazawa, Haruki" uniqKey="Kitazawa H" first="Haruki" last="Kitazawa">Haruki Kitazawa</name><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Villena, Julio" sort="Villena, Julio" uniqKey="Villena J" first="Julio" last="Villena">Julio Villena</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman</wicri:regionArea><wicri:noRegion>Tucuman</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in immunology</title><idno type="eISSN">1664-3224</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The oral administration of <i>Lacticaseibacillus rhamnosus</i> CRL1505 differentially modulates the respiratory innate antiviral immune response triggered by Toll-like receptor 3 (TLR3) activation in infant mice, improving the resistance to Respiratory Syncytial Virus (RSV) infection. In this work, by using macrophages depletion experiments and a detailed study of their production of cytokines and antiviral factors we clearly demonstrated the key role of this immune cell population in the improvement of both viral elimination and the protection against lung tissue damage induced by the CRL1505 strain. Orally administered <i>L. rhamnosus</i> CRL1505 activated alveolar macrophages and enhanced their ability to produce type I interferons (IFNs) and IFN-γ in response to RSV infection. Moreover, an increased expression of <i>IFNAR1, Mx2, OAS1, OAS2, RNAseL</i>, and <i>IFITM3</i> was observed in alveolar macrophages after the oral treatment with <i>L. rhamnosus</i> CRL1505, which was consistent with the enhanced RSV clearance. The depletion of alveolar macrophages by the time of <i>L. rhamnosus</i> CRL1505 administration abolished the ability of infant mice to produce increased levels of IL-10 in response to RSV infection. However, no improvement in IL-10 production was observed when primary cultures of alveolar macrophages obtained from CRL1505-treated mice were analyzed. Of note, alveolar macrophages from the CRL1505 group had an increased production of IL-6 and IL-27 suggesting that these cells may play an important role in limiting inflammation and protecting lung function during RSV infection, by increasing the maturation and activation of Treg cells and their subsequent production of IL-10. In addition, we provided evidence of the important role of CD4<sup>+</sup> cells and IFN-γ in the activation of alveolar macrophages highlighting a putative pathway through which the intestinal and respiratory mucosa are communicated under the influence of <i>L. rhamnosus</i> CRL1505.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">33133080</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1664-3224</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in immunology</Title><ISOAbbreviation>Front Immunol</ISOAbbreviation></Journal><ArticleTitle>Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered <i>Lacticaseibacillus rhamnosus</i> CRL1505.</ArticleTitle><Pagination><MedlinePgn>568636</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fimmu.2020.568636</ELocationID><Abstract><AbstractText>The oral administration of <i>Lacticaseibacillus rhamnosus</i> CRL1505 differentially modulates the respiratory innate antiviral immune response triggered by Toll-like receptor 3 (TLR3) activation in infant mice, improving the resistance to Respiratory Syncytial Virus (RSV) infection. In this work, by using macrophages depletion experiments and a detailed study of their production of cytokines and antiviral factors we clearly demonstrated the key role of this immune cell population in the improvement of both viral elimination and the protection against lung tissue damage induced by the CRL1505 strain. Orally administered <i>L. rhamnosus</i> CRL1505 activated alveolar macrophages and enhanced their ability to produce type I interferons (IFNs) and IFN-γ in response to RSV infection. Moreover, an increased expression of <i>IFNAR1, Mx2, OAS1, OAS2, RNAseL</i>, and <i>IFITM3</i> was observed in alveolar macrophages after the oral treatment with <i>L. rhamnosus</i> CRL1505, which was consistent with the enhanced RSV clearance. The depletion of alveolar macrophages by the time of <i>L. rhamnosus</i> CRL1505 administration abolished the ability of infant mice to produce increased levels of IL-10 in response to RSV infection. However, no improvement in IL-10 production was observed when primary cultures of alveolar macrophages obtained from CRL1505-treated mice were analyzed. Of note, alveolar macrophages from the CRL1505 group had an increased production of IL-6 and IL-27 suggesting that these cells may play an important role in limiting inflammation and protecting lung function during RSV infection, by increasing the maturation and activation of Treg cells and their subsequent production of IL-10. In addition, we provided evidence of the important role of CD4<sup>+</sup> cells and IFN-γ in the activation of alveolar macrophages highlighting a putative pathway through which the intestinal and respiratory mucosa are communicated under the influence of <i>L. rhamnosus</i> CRL1505.</AbstractText><CopyrightInformation>Copyright © 2020 Garcia-Castillo, Tomokiyo, Raya Tonetti, Islam, Takahashi, Kitazawa and Villena.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Garcia-Castillo</LastName><ForeName>Valeria</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tomokiyo</LastName><ForeName>Mikado</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raya Tonetti</LastName><ForeName>Fernanda</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Islam</LastName><ForeName>Md Aminul</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takahashi</LastName><ForeName>Hideki</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kitazawa</LastName><ForeName>Haruki</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Villena</LastName><ForeName>Julio</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Immunol</MedlineTA><NlmUniqueID>101560960</NlmUniqueID><ISSNLinking>1664-3224</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lacticaseibacillus rhamnosus CRL1505</Keyword><Keyword MajorTopicYN="N">Respiratory Syncytial Virus</Keyword><Keyword MajorTopicYN="N">TLR3</Keyword><Keyword MajorTopicYN="N">alveolar macrophages</Keyword><Keyword MajorTopicYN="N">immunobiotics</Keyword><Keyword MajorTopicYN="N">viral immunity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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